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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD66b (CEACAM-8, CEA-related cell adhesion molecule 8, CGM6, CEA gene family member 6, NCA-95, Nonspecific crossreacting antigen 95)

Anti -CD66b (CEACAM-8, CEA-related cell adhesion molecule 8, CGM6, CEA gene family member
6, NCA-95, Nonspecific crossreacting antigen 95)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
CD66b is a 95-100kD GPI anchored molecule and is expressed strongly by mature granulocytes and metamyelocytes but weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes.
Catalog #C2421-03
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionsFlow Cytometry: 1:50-1:100. Use 10ul labels 5x10e5 cells or 100ul whole blood.
Immunohistochemistry (Frozen): 1:25-1:100.
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsI1903-13A: IgG, F(abí)2, X-Adsorbed (PE) Pab: Gt xMo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt xMo
Hybridoma
Spleen cells from immunized BALB/c mice were fused with cells of the mouse MOPC 315-43 myeloma cell line.
Recommended Negative ControlsI1904-78R: IgG1 Murine Negative Control
Recommended Positive Control TissueBone Marrow
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H1637
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenCML leukocytes
SpecificityRecognizes the CGM6 gene product of the carcinoembryonic gene family. CD66b is expressed strongly by mature granulocytes and metamyelocytes but weakly by bone marrow myelocytes. CD66b is not expressed by peripheral blood monocytes or lymphocytes. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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