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You are here:Home » Antibodies » Abs to Receptors, Scavenger » Anti -CD68 (MACROSIALIN, DKFZp686M18236, Macrophage antigen, SCARD1, Scavenger receptor class D member1)

Anti -CD68 (MACROSIALIN, DKFZp686M18236, Macrophage antigen, SCARD1, Scavenger receptor
class D member1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH
C2423-02 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. C2423-02 reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.
Catalog #C2423-02
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (Formalin/paraffin): 1:200-1:400 for 20 minutes at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 20 minutes followed by colling at RT for 20 minutes.
Optimal dilution determined by the researcher.
Positive ControlTonsil, lymph node or spleen
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No3F103
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenSubcellular fraction of human alveolar macrophages. Cellular Localization: Cytoplasmic
SpecificityRecognizes the human glycoprotein of 110kD, which is identified as CD68. Species Crossreactivity: monkey, feline and rat. Does not react with porcine, canine or chicken
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDKFZp686M18236, Macrophage antigen CD68 (microsialin), GP110, SCARD1, Scavenger receptor class D member1


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