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You are here:Home » Antibodies » Abs to Receptors, Scavenger » Anti -CD68 (MACROSIALIN, DKFZp686M18236, Macrophage antigen, SCARD1, Scavenger receptor class D member1) (FITC)

Anti -CD68 (MACROSIALIN, DKFZp686M18236, Macrophage antigen, SCARD1, Scavenger receptor
class D member1) (FITC)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homolog of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (LAMP) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization. CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354 amino acids) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Catalog #C2423-05F
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required for this application.
Optimal dilution determined by the researcher
Recommended Negative ControlI1904-82E1: IgG2a, Rat Negative Control (FITC)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A326
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified Concanavalin A acceptor glycoprotein from P815 cell line
SpecificityRecognizes mouse CD68. Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. This antibody detects surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesDKFZp686M18236, Macrophage antigen CD68 (microsialin), GP110, SCARD1, Scavenger receptor class D member1


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