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You are here:Home » Antibodies » Abs to Receptors, Scavenger » Anti -CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1)

Anti -CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin,
Scavenger Receptor Class D Member 1, SCARD1)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF RIA
CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization. CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354 amino acids) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.
Catalog #C2423-05N1
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, RIA and Western Blot. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required.
Immunohistochemistry (Paraffin): 1:100. Requires protein digestion pre-treatment of paraffin sections (e.g. trypsin or pronase)
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
HybridomaSpleen cells from BLAB/c mice were fused with cells of the SP2/0-Ag-14 mouse myeloma cell line
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B1909
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat spleen cells
SpecificityRecognizes rat CD68 at ~110kD expressed predominantly on the lysosomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages and weakly by peripheral blood granulocytes.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCD68 Antigen, CD68 Molecule, Monocytes/Macrophages


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