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You are here:Home » Antibodies » Abs to Receptors, Scavenger » Anti -CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1)

Anti -CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin,
Scavenger Receptor Class D Member 1, SCARD1)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP IH FC IF
Macrosialin is a heavily glycosylated transmembrane protein of 87-115kD, which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablisation.
Catalog #C2423-05P
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Membrane permeabilization is required
Immunohistochemistry: Frozen sections
Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. (See reference 13, Devey et al, Mol. Ther. 2009)
Western Blot: Non-reducing conditions recommended
Optimal dilutions to be determined by the researcher.
Recommended Negative ControlI1904-82D1: IgG2a, Rat Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10B1906
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified glycoproteins from P815 cell line
SpecificityRecognizes mouse CD68. Detects surface macrosialin at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCD68 Antigen, CD68 Molecule, Monocytes/Macrophages


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