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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD70 (Tnfsf7, CD27 Ligand)

Anti -CD70 (Tnfsf7, CD27 Ligand)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IC
CD27 Ligand is a member of the tumor necrosis factor superfamily (TNFSF7) and is also designated CD70. CD27 Ligand is a type II transmembrane protein expressed by activated T and B cells. It binds to CD27 on mature T cells and a subset of thymocytes. CD27 Ligand induces the proliferation of costimulated T cells and enhances the generation of cytolytic T cells.
Catalog #C2425-01C
SourceTissue culture supernatant
ApplicationsSuitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended DilutionsImmunocytochemistry: 8-25ug/ml labels immersion fixed human peripheral blood mononuclear cells
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H76
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa45-193 from human CD27 Ligand expressed in NS0 cells (AAA36175).
SpecificityRecognizes human CD27 Ligand. In Western Blot, shows no crossreactivity with recombinant mouse CD27 Ligand, recombinant human CD30 Ligand or recombinant human CD40 Ligand.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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