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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IC

CD27 Ligand is a member of the tumor necrosis factor superfamily (TNFSF7) and is also designated CD70. CD27 Ligand is a type II transmembrane protein expressed by activated T and B cells. It binds to CD27 on mature T cells and a subset of thymocytes. CD27 Ligand induces the proliferation of costimulated T cells and enhances the generation of cytolytic T cells.
Catalog #	C2425-01C
Source	Tissue culture supernatant
Applications	Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilutions	Immunocytochemistry: 8-25ug/ml labels immersion fixed human peripheral blood mononuclear cells
	Western Blot: 1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	7H76
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant protein corresponding to human CD27 Ligand expressed in NS0 (Gln45-Pro193, Accession #AAA36175).
Specificity	Recognizes human CD27 Ligand. In Western Blot, shows no crossreactivity with recombinant mouse CD27 Ligand, recombinant human CD30 Ligand or recombinant human CD40 Ligand.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.