| Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family. |
|
| Catalog # | C2425-01M |
| Applications | Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. |
| Functional Application | Antibody inhibits T cell proliferation induced by dendritic cells. |
| Recommended Dilutions | Flow Cytometry: 10ug/ml. Use 80ul to label 5x10e5 Raji cells. |
| Immunohistochemistry: Frozen sections |
| Optimal dilutions to be determined by the researcher. |
| Recommended Negative Control | I1904-78R2: IgG1 Murine Negative Control (Biotin) |
| Flow Cytometry Protocol | 1. 5x10e5 cultured Raji cells were washed, then incubated 45 minutes on ice with 80ul of C2425-01M at 10ug/ml. |
| 2. Cells were then washed twice and incubated for 45 minutes with S7973-88P: Streptavidin (PE). |
| 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. |
| 5. Binding was blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of anti-CD70 (C2425-02). |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 7H173 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human WM-1 (Waldenström's macroglobulinemia) cell line. |
| Specificity | Recognizes the human CD70 molecule of ~30kD. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
