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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD70 (Tnfsf7, CD27 Ligand) (Biotin)

Anti -CD70 (Tnfsf7, CD27 Ligand) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH IC FC
Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family.
Catalog #C2425-01M
ApplicationsSuitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Functional ApplicationAntibody inhibits T cell proliferation induced by dendritic cells.
Recommended DilutionsFlow Cytometry: 10ug/ml. Use 80ul to label 5x10e5 Raji cells.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Recommended Negative ControlI1904-78R2: IgG1 Murine Negative Control (Biotin)
Flow Cytometry Protocol1. 5x10e5 cultured Raji cells were washed, then incubated 45 minutes on ice with 80ul of C2425-01M at 10ug/ml.
2. Cells were then washed twice and incubated for 45 minutes with S7973-88P: Streptavidin (PE).
3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration.
5. Binding was blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of anti-CD70 (C2425-02).
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H173
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman WM-1 (Waldenström's macroglobulinemia) cell line.
SpecificityRecognizes the human CD70 molecule of ~30kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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