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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E IH IC FC

Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family.
Catalog #	C2425-01P
Applications	Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:50 (or use 1.6ul of concentrated stock per 5x10e5 cell test).
	Optimal dilutions to be determined by the researcher.
FACS Protocol	1. Five x 10e5 cultured Raji human tumor cells were washed and incubated for 45 minutes on ice with 80ul of C2425-01P at a 1:50 dilution (10ug/ml).
	2. Cells were washed three times, fixed and analyzed by FACS.
	3. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a I1904-79D IgG1 Murine Negative Control (PE) at a similar concentration.
	4. Binding was blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of C2425-01L.
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	7H173
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human WM-1 (Waldenström's macroglobulinemia) cell line.
Specificity	Recognizes human CD70 molecule at ~30kD. Inhibits T cell proliferation induced by dendritic cells.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.