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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD70 (Tnfsf7, CD27 Ligand) (PE)

Anti -CD70 (Tnfsf7, CD27 Ligand) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH IC FC
Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family.
Catalog #C2425-01P
ApplicationsSuitable for use in FLISA, Flow Cytometry, Immunocytochemistry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50 (or use 1.6ul of concentrated stock per 5x10e5 cell test).
Optimal dilutions to be determined by the researcher.
FACS Protocol1. Five x 10e5 cultured Raji human tumor cells were washed and incubated for 45 minutes on ice with 80ul of C2425-01P at a 1:50 dilution (10ug/ml).
2. Cells were washed three times, fixed and analyzed by FACS.
3. Cells stained positive with a mean shift of 1.36 log10 fluorescent units when compared to a I1904-79D IgG1 Murine Negative Control (PE) at a similar concentration.
4. Binding was blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of C2425-01L.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No7H173
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman WM-1 (Waldenström's macroglobulinemia) cell line.
SpecificityRecognizes human CD70 molecule at ~30kD. Inhibits T cell proliferation induced by dendritic cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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