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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD79a (B-cell)

Anti -CD79a (B-cell)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IF
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines.
Catalog #C2431-10
ApplicationsSuitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 0.5-1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 0.5-1ug/ml for 30 minutes at RT. Requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlRaji cells, lymph nodes and tonsils.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No0.N.163
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C2431-10X.
PurityPurified by Protein G affinity chromatography.
ImmunogenSynthetic peptide consisting of aa (GTYQDVGSLNIADVQ) from human CD79a protein. Cellular Localization: Cell membrane.
SpecificityRecognizes human CD79a at ~47kD. Species Crossreactivity: monkey, equine, bovine, porcine, rabbit, guinea pig, rat, mouse, chicken (Plasma cells). Does not react with opossum
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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