| The affinity of the C2441-06L for rat Thy-1 is 3x10e9m-1 and for mouse Thy-1.1 is 3x10e8m-1 (1). The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T-Lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue. The molecule is a glycoprotein with 112aa which is homologous to immunoglobulin domains. The binding characteristics have been extensively studied. Demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5) and reported to induce glomerular nephritis in Wistar rats (8). |
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| Catalog # | C2441-06L |
| Applications | Suitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:50-1:100. 10ul labels 10e6 cells in 100ul. |
| Immunohistochemistry: Frozen sections |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | Goat anti-mouse IgG labeled with FITC (rat absorbed): |
| I1904-03A: IgG, X-Adsorbed (FITC) Pab Dk xMo |
| I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| Goat anti mouse IgG labeled with RPE (rat adsorbed) |
| I1903-12X: IgG, H&L, X-Adsorbed (PE) Pab Dk xMo |
| Recommended Negative Controls | I1904-78Y: IgG1, Murine Negative Control (For rat tissue) |
| Hybridoma | NS1 myeloma cells with spleen cells from Balb/c mice. |
| Source | Tissue culture supernatant |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1751 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Rat Thy 1 antigen |
| Specificity | Recognizes the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus C2441-06L reacts with Thy-1.1 mice e.g. AKR and FBV strains, but not Thy-1.2 mice e.g. CBA, BALB/c. Species Crossreactivity: rabbit, mouse, and guinea pig. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
