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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD90 (Thy-1)

Anti -CD90 (Thy-1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC
The affinity of the C2441-06L for rat Thy-1 is 3x10e9m-1 and for mouse Thy-1.1 is 3x10e8m-1 (1). The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T-Lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue. The molecule is a glycoprotein with 112aa which is homologous to immunoglobulin domains. The binding characteristics have been extensively studied. Demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (5) and reported to induce glomerular nephritis in Wistar rats (8).
Catalog #C2441-06L
ApplicationsSuitable for use in Immunohistochemistry, Western Blot, Immunoprecipitation and Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50-1:100. 10ul labels 10e6 cells in 100ul.
Immunohistochemistry: Frozen sections
Optimal working dilutions to be determined by researcher.
Recommended Secondary ReagentsGoat anti-mouse IgG labeled with FITC (rat absorbed):
I1904-03A: IgG, X-Adsorbed (FITC) Pab Dk xMo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
Goat anti mouse IgG labeled with RPE (rat adsorbed)
I1903-12X: IgG, H&L, X-Adsorbed (PE) Pab Dk xMo
Recommended Negative ControlsI1904-78Y: IgG1, Murine Negative Control (For rat tissue)
HybridomaNS1 myeloma cells with spleen cells from Balb/c mice.
Source Tissue culture supernatant
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H1751
HostMouse
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRat Thy 1 antigen
SpecificityRecognizes the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus C2441-06L reacts with Thy-1.1 mice e.g. AKR and FBV strains, but not Thy-1.2 mice e.g. CBA, BALB/c. Species Crossreactivity: rabbit, mouse, and guinea pig.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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