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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified B IP IH FC

Thy-1 was discovered in early 1960s during the search for heterologous antisera against mouse leukemia cells. It was originally named theta ( ) antigen, then Thy-1 (THYmocyte differentiation antigen 1) due to its prior identification in thymocytes (precursors of T cells in the thymus). The human homolog was isolated in 1980 as a 25kDa protein (p25) of T-lymphoblastoid cell line MOLT-3 binding with anti-monkey-thymocyte antisera. The conserved gene and its alleles Thy-1 has been conserved throughout vertebrate evolution and even in some invertebrates, with homologs described in many species like squid, frogs, chickens, mice, rats, dogs, and humans. The Thy-1 gene is located at human chromosome 11q22.3 (mouse chromosome 9qA5.1). Some believe that there may be a functional significance of both this gene and CD3 delta subunit (T3D) mapping to chromosome 11q in man and chromosome 9 in mouse, though there is no homology (in fact this speculation lead to its localization in chromosome 11q-the human chromosome region syntenic to mouse chromosome 9 which harbored T3D). In mouse there are two alleles Thy1.1 (Thy 1a, CD90.1) and Thy1.2 (Thy 1b, CD90.2) which differ by one amino acid at position 108 (arginine in Thy-1.1 and glutamine in Thy-1.2). Thy 1.2 is expressed by most strains of mice, whereas Thy1.1 is expressed by some like AKR/J and PL mouse strains.
Catalog #	C2441-85B
F(ab')2 Goat anti-rat IgG	RPE (mouse adsorbed)
Fusion Partners	Spleen cells from immunized Sprague-Dawley rats were fused with cells from the mouse SP2/0-Ag14 myeloma cell line.
Recommended Negative Controls	Rat IgG1 Negative Control
Applications	Flow Cytometry: 1:50-1:100
	Immunohistochemistry (frozen)
	Immunoprecipitation
	Western Blot
	Optimal working dilutions to be determined by researcher.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H3036
Host	Rat
Source	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	EL-4 cells.
Specificity	Recognizes the mouse Thy1.2 cell surface antigen and binds to Tcell lines (EL-4, 3A9), T lymphocytes and the WEH1-3 cell line. 3T3 fibroblasts are also stained by this antibody. This clone recognizes a 30kD glycoprotein in immunoprecipitation and Western blotting assays. The antibody strongly induces homotypic adhesion of Thy1.2 positive cell lines.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.