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Anti -CD100 (Leukocyte Semaphorin) (Biotin)
Anti -CD100 (Leukocyte Semaphorin) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC |
|
| Human CD100 is a transmembrane glycoprotein that belongs to the semaphorin gene family and is found on most hematopoietic cells except RBC, platelets and immature bone marrow cells. CD100 plays a key role in lymphocyte activation. | | | Catalog # | C2445-25B | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilution | Flow Cytometry: 1ug/ml | | Immunohistochemistry: Frozen sections | | Optimal dilutions to be determined by the researcher. | | Flow Cytometry Protocol | 1. 5x10e5 cultred human HPB-MLT cells were washed and preincubated for 45 minutes on ice with 80ul of C2445-25B at 1ug/ml. | | 2. Wash cells twice and incubate with Streptavidin (PE). | | 3. Wash cells three times, fix and analyze using FACS. | | 3. Cells were stained positive with a mean shift of 0.83 log10 flourescent units when compared to a mouse IgGM/Biotin negative control at a similar concentration. | | 4. Binding was blocked when pre-incubatde 10 minutes with 20ul of 0.5mg/ml CD100 (C2445-25A). | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgM | | Clone No | 7H103 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.5, 5% Glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | PHA stimulated human PBL | | Specificity | Recognizes human CD100 at ~150kD. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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