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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD100 (Leukocyte Semaphorin) (Biotin)

Anti -CD100 (Leukocyte Semaphorin) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
Human CD100 is a transmembrane glycoprotein that belongs to the semaphorin gene family and is found on most hematopoietic cells except RBC, platelets and immature bone marrow cells. CD100 plays a key role in lymphocyte activation.
Catalog #C2445-25B
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1ug/ml
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. 5x10e5 cultred human HPB-MLT cells were washed and preincubated for 45 minutes on ice with 80ul of C2445-25B at 1ug/ml.
2. Wash cells twice and incubate with Streptavidin (PE).
3. Wash cells three times, fix and analyze using FACS.
3. Cells were stained positive with a mean shift of 0.83 log10 flourescent units when compared to a mouse IgGM/Biotin negative control at a similar concentration.
4. Binding was blocked when pre-incubatde 10 minutes with 20ul of 0.5mg/ml CD100 (C2445-25A).
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgM
Clone No7H103
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 5% Glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenPHA stimulated human PBL
SpecificityRecognizes human CD100 at ~150kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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