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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH FC

CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.
Catalog #	C2445-30
Applications	Removal of sodium azide is recommended prior to use in functional assay.
	Immunohistochemistry: Frozen
	Immunoprecipitation
	Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Recommended Secondary Reagents	F(ab')2 Rabbit anti mouse IgG: FITC
	F(ab')2 Rabbit anti mouse IgG: RPE
Recommended Secondary Reagents F(ab')2 rabbit anti-mouse IgG	HRP conjugate
Recommended Negative Controls	Mouse IgG1 Negative Control
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4j29
Host	Mouse
Source	Human
Concentration	1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein G affinity chromatography from tissue culture supernatant
Immunogen	Human thymic clone B12
Specificity	Recognizes human CD101. Reported to inhibit allogeneic T-cell responses (1).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.