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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD101 (PE)

Anti -CD101 (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
CD101 is a 140kD homodimeric cell surface glycoprotein expressed primarily by monocytes, granulocytes, dendritic cells and activated T lymphocytes. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.
Catalog #C2445-30B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat -1:5. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: frozen samples
Optimal dilutions to be determined by the researcher.
Recommended Negative ControlsI1904-79D: IgG1 Murine Negative Control (PE)
Storage and StabilityLyophilized powder may be stored at 4°C. Reconstitute with ddH2O. Aliquot and store at 4°C. Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4j29
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman thymic clone B12
SpecificityRecognizes human CD101. Reported to inhibit allogeneic T-cell responses (1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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