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Anti -CD101 (EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, Gm1016, Gm734, Igsf2, IgSF2, Immunoglobulin superfamily member 2, LOC381460)
Anti -CD101 (EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, Gm1016, Gm734, Igsf2,
IgSF2, Immunoglobulin superfamily member 2, LOC381460)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
FC |
|
| IGSF2, also known as CD101, is an Ig-superfamily transmembrane protein. It is expressed on dendritic cells, Langerhans cells, granulocytes, and activated T cells. IGSF2 ligation is involved in T cell activation. Polymorphisms in IGSF2 are associated with susceptibility to type I diabetes. The extracellular domains of mouse and human IGSF2 share 70% amino acid sequence identity. | | | Catalog # | C2445-30C | | Hybridoma | Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified recombinant mouse IGSF2 (rmIGSF2; Accession # BAD97674) extracellular domain. | | Applications | Flow Cytometry: Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 L of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. | | Optimal dilutions should be determined by each laboratory for each application. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 7H22 | | Host | Rat | | Source | Mouse | | Concentration | As reported | | Form | Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | RmIGSF2 extracellular domian | | Specificity | Selected for its ability to detect IGSF2 on mouse splenocytes in flow cytometry. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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