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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD101 (EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, Gm1016, Gm734, Igsf2, IgSF2, Immunoglobulin superfamily member 2, LOC381460)

Anti -CD101 (EWI-101, Glu-Trp-Ile EWI motif-containing protein 101, Gm1016, Gm734, Igsf2,
IgSF2, Immunoglobulin superfamily member 2, LOC381460)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
IGSF2, also known as CD101, is an Ig-superfamily transmembrane protein. It is expressed on dendritic cells, Langerhans cells, granulocytes, and activated T cells. IGSF2 ligation is involved in T cell activation. Polymorphisms in IGSF2 are associated with susceptibility to type I diabetes. The extracellular domains of mouse and human IGSF2 share 70% amino acid sequence identity.
Catalog #C2445-30C
HybridomaProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified recombinant mouse IGSF2 (rmIGSF2; Accession # BAD97674) extracellular domain.
ApplicationsFlow Cytometry: Dilute this antibody to 25ug/ml and add 10 L of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200 L. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 L of a 25ug/ml stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No7H22
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenRmIGSF2 extracellular domian
SpecificitySelected for its ability to detect IGSF2 on mouse splenocytes in flow cytometry.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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