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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD102 (Intercellular Adhesion Molecule, ICAM-2)

Anti -CD102 (Intercellular Adhesion Molecule, ICAM-2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
CD102 is a glycosylated cell surface protein that is broadly expressed on most leukocytes and is strongly expressed by endothelial cells. CD102 interacts with the CD11a/CD18 (LFA-1). Reports suggest that the CD102 may play a role in lymphocyte re-circulation and T-cell activation.
Catalog #C2445-44C
Fusion Partners Cells from immunized BALB/c mice were fused with cells of the Murine P3X63Ag.653 myeloma cell line.
ApplicationsSuitable for use in Flow Cytometry, ELISA, Immunoprecipitation. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:100. Use 10ul of the suggested working dilution to label 10e6cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsF(ab')2 Rabbit anti mouse IgG:FITC
F(ab')2 Rabbit anti mouse IgG:RPE
ImmunohistologyRecommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:HRP
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A356
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Conjugated to R-Phycoerythrin (PE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
PurityPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
ImmunogenTransfected COS cells expressing ICAM-2 cDNA.
SpecificityRecognizes human CD102, also known as intercellular adhesion molecule-2 (ICAM-2).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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