| CD102 is a glycosylated cell surface protein that is broadly expressed on most leukocytes and is strongly expressed by endothelial cells. CD102 interacts with the CD11a/CD18 (LFA-1). Reports suggest that the CD102 may play a role in lymphocyte re-circulation and T-cell activation. |
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| Catalog # | C2445-44C |
| Fusion Partners | Cells from immunized BALB/c mice were fused with cells of the Murine P3X63Ag.653 myeloma cell line. |
| Applications | Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:100. Use 10ul of the suggested working dilution to label 10e6cells in 100ul. |
| Immunohistochemistry: Frozen |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Reagents | F(ab')2 Rabbit anti mouse IgG:FITC |
| F(ab')2 Rabbit anti mouse IgG:RPE |
| Immunohistology | Recommended Secondary Reagents: |
| F(ab')2 rabbit anti mouse IgG:HRP |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 6A356 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a lyophilized powder in PBS, pH 7.2, 1% BSA, 0.1% sodium azide. Conjugated to R-Phycoerythrin (PE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O. |
| Purity | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. |
| Immunogen | Transfected COS cells expressing ICAM-2 cDNA. |
| Specificity | Recognizes human CD102, also known as intercellular adhesion molecule-2 (ICAM-2). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
