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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD102 (CD102 Antigen, Intercellular Adhesion Molecule 2, ICAM 2, ICAM2, ICAM-2, Ly60) (Low Endotoxin)

Anti -CD102 (CD102 Antigen, Intercellular Adhesion Molecule 2, ICAM 2, ICAM2, ICAM-2,
Ly60) (Low Endotoxin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified IP IH FC
Murine CD102 is a glycosylated cell surface protein that is broadly expressed on most leukocytes and is constitutively expressed on endothelial cells. CD102 interacts with the CD11a/CD18 integrin LFA-1. Reports suggest that CD102 may play a role in lymphocyte re-circulation and T-cell activation.
Catalog #C2445-59
Applications
Suitable for use in Flow Cytometry, Immunohistochemistry (frozen), Functional Activity and Immunoprecipitation . Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50-1:200 Use 10ul to label 10e6 cells in 100ul.
Optimal dilution determined by the researcher
Endotoxin Level<0.01 EU/ug
HybridomaSpleen cells from immunized Lewis rats were fused with cells of the Mouse P3X63Ag8.653 myeloma cell line..
Recommended Secondary ReagentsF(ab')2 Goat anti rat IgG:FITC (mouse adsorbed)
F(ab')2 Goat anti rat IgG:RPE (mouse adsorbed)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A359
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenCOS cells transfected with murine ICAM-2.
SpecificityRecognizes murine intercellular adhesion molecule-2 (ICAM-2). Reported to inhibit interactions between CD102 and LFA-1(1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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