| Catalog # | C2445-63 |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry (frozen) and Immunoprecipitation. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 10ul of the suggested working dilution to label 10e6 cells in 100ul. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSO myeloma cell line. |
| Recommended Negative Control | I1904-78R: IgG1 Murine Negative Control |
| Recommended Secondary Reagents | I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo |
| I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1797 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.09% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Hairy cell leukaemia cells. |
| Specificity | Recognizes the human CD103 cell surface antigen, a glycoprotein expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes. It has recently been shown to be identical to the alpha E integrin. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
