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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH FC

Catalog #	C2445-63A
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry
	and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Flow Cytometry: 1:50-1:100 Use 10ul of the suggested working dilution to label 5x10e5 cells in 100ul.
	Immunohistochemistry (frozen): 1:50-1:100
	Optimal working dilutions to be determined by researcher.
Hybridoma	Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Recommended positive control tissue	Intestine.
Recommended Secondary Reagents	I1904-65W IgG, F(ab’)2 (HRP) Pab Rb xMo
	I1904-66C IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo
Recommended Negative Controls	I1904-83P1: IgG2a Murine Negative Control
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	3H1798
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human Intestinal lymphocyte.
Specificity	Recognizes the human CD103 cell surface antigen, also known as the alpha-E integrin. This reacts with various subsets of human intra-epithelial lymphocytes
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.