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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IP IH FC

CD103 is the integrin alphaE subunit which usually forms a heterodimer with the integrin beta7 subunit. It is expressed on intraepithelial lymphocytes and 1-2% of peripheral blood lymphocytes. CD103 binds E-cadherin on epithelial cells. This interaction may be of importance in the homing and retention of CD103+ lymphocytes in the intestinal epithelium (1-4). PE/Cy5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
Catalog #	C2445-63J
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 5ul/10e6 cells
	Immunohistochemistry: Frozen sections
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5K293
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with PE/CY5.
Purity	Purified
Specificity	Recognizes human CD103.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.