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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP IH FC

Catalog #	C2446-01
Applications	Inhibition of cell attachment: inhibits SCC9 cell adhesion to human and EHS laminin, but not to laminin GD-2 peptide, type IV collagen or fibronectin.
	Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
	Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
	Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
	Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells.
Storage and Handling	Maintain at 2–8°C in undiluted aliquots for up to 12 months
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	2Q792
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in 0.02M PB pH 7.6, 0.25M sodium chloride containing 0.1% sodium azide
Purity	Purified immunoglobulin from Protein A Sepharose chromatography
Immunogen	Human squamous cell carcinoma cells (SCC9)
Specificity	Human b4 integrin (CD104)
Important Note	This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.