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You are here:Home » Antibodies » Abs to Integrin » Anti -CD104 (Integrin beta4)

Anti -CD104 (Integrin beta4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
Catalog #C2446-01E
ApplicationsSuitable for use in Immunohistochemistry, Flow Cytometry, Inhibition of Cell Attchment and Immunoprecipitation. Other applications not tested.
Recommended DilutionsImmunohistochemistry: On acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue.
Flow Cytometry: Stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.
Inhibition of Cell Attachment: Inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptide, but not to type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
Immunoprecipitation: Precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No2Q793
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS pH 7.6, 0.1% sodium azide
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman squamous cell carcinoma cells (SCC9)
SpecificityRecognizes human b4 integrin (CD104)
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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