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You are here:Home » Antibodies » Antibodies-CD Markers » Anti -CD104 (Integrin beta4)

Anti -CD104 (Integrin beta4)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IC FC
Integrin beta 4 (also known as GP150 and CD104) is a 150-200kD member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683aa extracellular domain (ECD) (aa28-710) and a large 1089aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84aa substitution for the 84aa between aa621-704. A second shows a 144aa substitution for the first 704aa. Human Integrin beta4 ECD shares 88% sequence identity with mouse Integrin beta 4 ECD.
Catalog #C2446-01F
ApplicationsSuitable for use in Direct ELISA, Western Blot, Immunocytochemistry and Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and 10ng/lane under reducing conditions.
Immunocytochemistry: 10ug/ml detects Integrin B4 in human A431 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 min. at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 min. at RT. After blocking, cells were incubated with diluted C2446-01F overnight at 4°C followed by Rhodamine RedTM-coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well.
Flow Cytometry: 50ug/ml. Add 10ul to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by affinity chromatography
ImmunogenRecombinant protein corresponding to aa28-710 from human CD104 extracellular domain, expressed in CHO cells (P16144).
SpecificityRecognizes human Integrin B4. Species sequence homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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