Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to CD Markers » Anti -CD104 (Integrin beta4)

Anti -CD104 (Integrin beta4)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IC FC
Integrin beta 4 (also known as GP150 and CD104) is a 150-200kD member of the Integrin beta family. It forms noncovalent heterodimers with Integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human Integrin beta 4 is a type I transmembrane glycoprotein that is 1795 amino acids (aa) in length. It contains a 683 aa extracellular domain (ECD) (aa # 28-710) and a large 1089 aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84 aa substitution for the 84 aa between aa # 621-704. A second shows a 144 aa substitution for the first 704 amino acids. Human Integrin beta4 ECD shares 88% sequence identity with mouse Integrin beta 4 ECD.
Catalog #C2446-01F
ApplicationsSuitable for use in ELISA, Western Blot, Immunocytochemistry and Flow Cytometry. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and 10ng/lane under reducing conditions.
Immunocytochemistry: 10ug/ml detects Integrin B4 in human A431 cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 min. at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 min. at RT. After blocking, cells were incubated with diluted C2446-01F overnight at 4°C followed by Rhodamine RedTM-coupled anti-sheep IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1% BSA.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well.
Flow cytometry: 50ug/ml. Add 10ul to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Optimal dilutions should be determined by each laboratory for each application.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.
PurityPurified by Immunoaffinity chromatography.
ImmunogenCHO cell-derived rhIntegrin B4 extracellular domain, aa28-710.
SpecificityRecognizes human Integrin B4. Species sequence homology
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links