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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD104 (Integrin beta4)

Anti -CD104 (Integrin beta4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IC FC
CD104 (also GP150 and Integrin beta 4) is a 150-200kD member of the integrin beta family. It forms noncovalent heterodimers with integrin alpha 6 and participates in the formation of epithelial hemidesmosomes. Human integrin beta 4 is a type I transmembrane glycoptotein that is 1795 amino acids (aa) in length. It contains a 683aa extracellular domain (ECD) (aa28-710) and a large 1089aa cytoplasmic region. Two ECD alternate splice forms may exist. One shows an 84aa substitution for the 84aa between aa21-704. A second shows a 144aa substitution for the first 704aa. Human beta 4 ECD shares 88% aa sequence identity with mouse beta4 ECD.
Catalog #C2446-01H
ApplicationsSuitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Other applications not tested.
Recommended DilutionsWestern Blot: 1-2ug/ml; Using a colorimetric detection system, the detection limit for recombinant human Integrin 4 is ~25ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection will increase sensitivity by 5-50 fold.
Flow Cytometry: 0.2mg/ml; 10ul labels 1-2.5x10e5 human A431 cells in a total reaction volume <200ul.
Immunocytochemistry: 10ug/ml detects CD104 in A431 human epidermoid carcinoma cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted C2446-01H overnight at 4ºC followed by anti-mouse IgG (FITC) at RT in the dark for 1 hour. Cells were washed with PBS, 0.1% BSA between each step.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No7H253
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa28-710 of human Integrin Beta 4 expressed in CHO cells (Accession # P16144).
SpecificityRecognizes human CD104 (Integrin beta4). Does not cross react with recombinant human Integrin beta1, beta2, beta3, beta5, beta6, beta8 or recombinant mouse Integrin beta4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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