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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B FC

Catalog #	C2446-02A
Applications	Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution	Flow Cytometry: 10ug/ml. Use 80ul to label 5x10e5 cells.
	Optimal dilutions to be determined by the researcher.
FACS Protocol	1. Five x10e5 cultured UM-SCC (Squamous Cell Carcinoma) cells were harvested by trypsinization.
	2. Five x10e5 cells per tube were washed and pre incubated ~ 5 minutes with 20ul of 250ug/ml of human IgG.
	3. Incubate 5 x10e5 cells on ice for 45 minutes with 80ul of C2446-02A at 10ug/ml.
	4. Cells were washed twice and incubated with IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo.
	5. Washed 3x, fixed and analyzed on a BD FACS Calibur.
	6.. Cells stained positive with a mean shift of 1.78 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7H228
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS pH 7.5, 0.5mg/ml gentamicin sulfate, <1% Bovine Immunoglobulin.
Purity	Purified by Protein A affinity chromatography.
Immunogen	UM-SCC-1, human squamous cell carcinoma.
Specificity	Recognizes the human CD104 molecule at ~ 220kD (non-reduced). Partially blocks binding to laminin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.