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C2446-02A Mouse Anti-CD104 (Integrin beta4)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a
Clone Number
7H228
Grade
Affinity Purified
Applications
FC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 10ug/ml. Use 80ul to label 5x10e5 cells. Optimal dilutions to be determined by the researcher.
FACS Protocol
1. Five x10e5 cultured UM-SCC (Squamous Cell Carcinoma) cells were harvested by trypsinization. 2. Five x10e5 cells per tube were washed and pre incubated ~ 5 minutes with 20ul of 250ug/ml of human IgG. 3. Incubate 5 x10e5 cells on ice for 45 minutes with 80ul of C2446-02A at 10ug/ml. 4. Cells were washed twice and incubated with IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo. 5. Washed 3x, fixed and analyzed on a BD FACS Calibur. 6.. Cells stained positive with a mean shift of 1.78 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
UM-SCC-1, human squamous cell carcinoma.
Form
Supplied as a liquid in PBS pH 7.5, 0.5mg/ml gentamicin sulfate, <1% Bovine Immunoglobulin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the human CD104 molecule at ~ 220kD (non-reduced). Partially blocks binding to laminin.
References
1. K.A. Kimmel and T.E. Carey, Cancer Res (1986) 46: 3614-3623. 2. C. VanWaes, et al, Cancer Res (1991) 51: 2395-2402. 3. Leukocyte Typing V (1995) S.F. Schlossman, et al, (eds.), Oxford University Press, NY. p. 1655-1663, 1667-1668.
USBio References
No references available
Conjugates
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