| Human beta4 integrin is also known as CD104. It is a 205kD glycoprotein. It associates with the alpha6 integrin to form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, Schwann cells and various tumor cell lines. |
|
| Catalog # | C2446-05 |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blot. Not suitable for use in Immunohistochemistry paraffin sections. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 1:25-1:100. Use 10ul of the optimal working dilution to label 10e6 cells in 100ul. |
| Immunohistochemistry (Frozen): 1:500-1:1000 |
| Optimal working dilutions to be determined by researcher. |
| Recommended Secondary Reagents | FC |
| I1904-66A: IgG, F(ab’)2, X-Adsorbed (FITC) Pab Rb xMo |
| Recommended Secondary Reagents | IHC |
| I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo |
| Recommended Positive Control | IHC |
| Human Tonsil. |
| Recommended Negative Control | I1904-78M: Mouse IgG1 Negative Control |
| Hybridoma | Spleen cells from immunized BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol |
| (40-50%) and aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1804 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Purified alpha6 beta4 integrin from A431 cells. |
| Specificity | Recognizes the human beta4 integrin. Recognizes an extracellular epitope on the CD104 molecule. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
