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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD105 (Endoglin, gp160, TGF beta1,3 Receptor)

Anti -CD105 (Endoglin, gp160, TGF beta1,3 Receptor)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IP IH FC
Endoglin is an endothelial homodimeric membrane antigen containing the tripeptide sequence RGD which is a recognition motif for adhesion receptors of the integrin family. CD105 is a Type I transmembrane protein and exists as an O-and N-glycosylated homodimer. Endoglin is a TGF beta-binding glycoprotein that is most abundantly expressed on endothelial cells in tissues with in ammatory or in ammatory-like characteristics, such as the skin lesions in psoriasis. Proliferating endothelial cells in granulation tissue and in tumors (e.g. melanoma of the skin) also exhibit enhanced expression of endoglin. In addition, a marked staining of syncytiotrophoblasts in placenta is observed. CD105 binds to TGF-b1 and b3 with high affinity but not TGF-b2.
Catalog #C2446-50A
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: 1:10-1:50 Fresh frozen or acetone fixed sections. Not suitable for paraffin embedded sections.
Flow Cytometry: 1ug per 10e6 cells in 100ul.
Western Blot: 1:500--1:1000. 90kD band observed under reducing condtions. Membrane preparations recommended, mouse vascular endothelial cells recommended as a positive control.
Immunoprecipitation: Use rabbit anti-rat secondary antibody, followed by Protein A or anti-rat beads for recovery.
Optimal dilutions to be determined by the researcher.
SourceTissue culture supernatant
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No2Q1707
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in pH 8.2, 100mM borate buffered saline. No preservatives added.
PurityPurified by affinity chromatography.
ImmunogenMouse CD105 (Endoglin).
SpecificityRecognizes mouse CD105.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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