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Anti -CD105 (Endoglin, gp160, TGF beta1,3 Receptor)
Anti -CD105 (Endoglin, gp160, TGF beta1,3 Receptor)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IH FC |
|
| Human CD105 (endoglin) is a homodimeric membrane glycoprotein virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity. | | | Catalog # | C2446-50E | | Applications | Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Flow cytometry: 10ug/ml | | Immunohistochemistry: frozen sections | | Optimal dilutions to be determined by the researcher. | | Flow cyytometry Protocol | 1. Five x 10e5 cultured Nalm-6 human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2446-50E at a concentration of 10ug/ml. | | 2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC | | 3. After which they were washed three times, fixed and analyzed by FACS. | | 4. Cells stained positive with a mean shift of 0.79 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 7H217 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml Gentamicin Sulfate. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Membrane preparation of human B-lineage leukemia cells | | Specificity | Recognizes human CD105 homodimer with a molecular weight of ~160kD. Antibody will augment binding of TGF-b1 to CD105 expressing leukemia cells. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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