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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD105 (Endoglin, gp160, TGF beta1,3 Receptor)

Anti -CD105 (Endoglin, gp160, TGF beta1,3 Receptor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Human CD105 (endoglin) is a homodimeric membrane glycoprotein virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity.
Catalog #C2446-50E
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionFlow cytometry: 10ug/ml
Immunohistochemistry: frozen sections
Optimal dilutions to be determined by the researcher.
Flow cyytometry Protocol1. Five x 10e5 cultured Nalm-6 human tumor cells were washed and incubated 45 minutes on ice with 80ul of C2446-50E at a concentration of 10ug/ml.
2. Cells were washed twice and incubated with secondary reagent Goat anti-Mouse IgG/FITC
3. After which they were washed three times, fixed and analyzed by FACS.
4. Cells stained positive with a mean shift of 0.79 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No7H217
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 0.5mg/ml Gentamicin Sulfate.
PurityPurified by Protein A affinity chromatography.
ImmunogenMembrane preparation of human B-lineage leukemia cells
SpecificityRecognizes human CD105 homodimer with a molecular weight of ~160kD. Antibody will augment binding of TGF-b1 to CD105 expressing leukemia cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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