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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD105 (Endoglin, gp160, TGF beta1,3 Receptor) (Biotin)

Anti -CD105 (Endoglin, gp160, TGF beta1,3 Receptor) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Human CD105 (endoglin) is a homodimeric membrane glycoprotein virtually restricted to endothelial cells, and present on some leukemia cells and minor subsets of bone marrow cells. CD105 binds to TGF-b1 and b3 with high affinity. Antibody will augment binding of TGF-b1 to CD105 expressing leukemia cells
Catalog #C2446-50E1
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Flow Cytometry Protocol1. 5x10e5 Nalm-6 were washed and incubated 45 minutes on ice with 80ul of C2446-50E1 at 5ug/ml.
2. Cells were washed twice and incubated with secondary reagent S7973-88P, Streptavidin/R-Phycoerythrin, after which they were washed three times fixed and analyzed using a FACS.
3. Cells stained positive with a mean shift of 1.81 log10 fluorescent units when compared to I1904-78R2 IgG1/biotin Murine Negative Control at a similar concentration.
4. Binding was 99% blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml C2446-50E, CD105.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No7H217
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.5, 5% Glycerol, 0.2% BSA, 0.04% and 0.04% sodium azide.. Labeled with biotin.
PurityPurified by Protein A affinity chromatography.
ImmunogenMembrane preparation of human B-lineage leukemia cells
SpecificityRecognizes a CD105 homodimer with a MW of ~160kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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