| CD105 is also known as endoglin. It is a glycoprotein homodimer of 95kD subunits. CD105 is a cell surface antigen. It is expressed by endothelial cells, activated monocytes and some leukemia cells. |
|
| Catalog # | C2446-55 |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 1:25. Use 10ul to label 1x10e6 cells in 100ul. |
| Immunohistochemistry: Frozen. Not suitable for use on paraffin sections. |
| Optimal working dilutions to be determined by researcher. |
| Suggested Secondary Antibodies | I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo |
| I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo |
| I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo |
| Suggested Negative Controls | I1904-78R: IgG1 Murine Negative Control |
| Hybridoma | P3/NS1/1-AG4-1 myeloma cells with spleen cells from Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1805 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Partially purified cell membrane antigens from fresh leukaemia cells. |
| Specificity | Recognizes the human CD105. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
