| CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) is expressed on activated endothelial cells and on some tissue macrophages, bone marrow fibroblasts and myoblasts. VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. |
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| Catalog # | C2446-74C |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Immunoprecipitation. Other applications have not been tested. |
| Recommended Dilution | Flow Cytometry: 1:5-1:25 |
| Immunohistochemistry: Frozen sections |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Reagents | I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo |
| I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo |
| I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo |
| F(ab')2 rabbit anti mouse IgG (PE) |
| Recommended Negative Control | I1904-78R: Mouse IgG1 Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1814 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodIum azide, before the addition of glycerol to 40%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | CD106 |
| Specificity | Recognizes human VCAM-1, a 110kD molecule whose ligand is VLA4. Inhibits cellular adhesion mediated by VCAM-1. Removal of sodium azide is recommended prior to use in functional assays. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
