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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110)

Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP IH FC
CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) is expressed on activated endothelial cells and on some tissue macrophages, bone marrow fibroblasts and myoblasts. VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines.
Catalog #C2446-74C
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionFlow Cytometry: 1:5-1:25
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Recommended Secondary ReagentsI1904-65W: IgG, F(abí)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(abí)2, X-Adsorbed (HRP) Pab RbxMo
I1904-66A: IgG, F(abí)2 (FITC) Pab Rb xMo
F(ab')2 rabbit anti mouse IgG (PE)
Recommended Negative ControlI1904-78R: Mouse IgG1 Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H1814
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.09% sodIum azide, before the addition of glycerol to 40%.
PurityPurified by Protein A affinity chromatography.
ImmunogenCD106
SpecificityRecognizes human VCAM-1, a 110kD molecule whose ligand is VLA4. Inhibits cellular adhesion mediated by VCAM-1. Removal of sodium azide is recommended prior to use in functional assays.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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