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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110)

Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC
VCAM-1, the vascular cell adhesion molecule, is a member of the immunoglobulin superfamily, C2 subset. The antigen is induced on human endothelial cells by TNF-alpha, IL-1, IFN-gamma or endotoxin. The ligands for VCAM-1 are integrin alpha4, beta1 (VLA-4) and integrin alpha4, beta7. These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes.
Catalog #C2446-77A
ApplicationsUseful for staining of VCAM-1 expressing endothelial cells. It permits staining of in vitro cultured cells and frozen tissue sections. Furthermore the antibody can be used for Flow Cytometry, immunoassays and Immunoprecipitation.
Recommended DilutionFlow Cytometry: 1:10
Immunohistology: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde, tissue sections are advised to be fixed for 10 min in pure acetone and followed by incubation for 10 min in chloroform. Incubation with a pretested dilution of the antibody is advised to be followed by a biotin conjugated anti-murine Ig and a further incubation with an enzyme (alkaline phosphatase) conjugated streptavidin. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1ng/ml), IL-1 (100U/ml) or LPS (1ug/ml) in tissue culture medium at 37°C. As negative control it is advised to use a control murine IgG1 antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A518 (1G11B1)
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.
PurityPurified by Protein G affinity chromatography.
SpecificityReacts with human VCAM-1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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