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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (Biotin)

Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH IC FC
VCAM-1, the vascular cell adhesion molecule, is a member of the immunoglobulin superfamily, C2 subset. The antigen is induced on human endothelial cells by TNF-alpha, IL-1, IFN-gamma or endotoxin. The ligands for VCAM-1 are integrin alpha4, beta1 (VLA-4) and integrin alpha4, beta7. These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes.
Catalog #C2446-77B
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:10
Immunohistochemistry (Frozen): 1:10. Fix for 10 min in pure acetone and then incubate for 10 min in chloroform. Incubation with a pretested dilution of C2446-77B is advised to be followed an enzyme (alkaline phosphatase) conjugated streptavidin.
Immunocytochemistry: 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1ng/ml), IL-1 (100U/ml) or LPS (1ug/ml) in tissue culture medium at 37°C.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A518 (1G11B1)
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenHuman CD106.
SpecificityRecognizes human CD106.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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