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Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (Biotin)
Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E IH IC FC |
|
| VCAM-1, the vascular cell adhesion molecule, is a member of the immunoglobulin superfamily, C2 subset. The antigen is induced on human endothelial cells by TNF-alpha, IL-1, IFN-gamma or endotoxin. The ligands for VCAM-1 are integrin alpha4, beta1 (VLA-4) and integrin alpha4, beta7. These integrins are constitutively expressed by thymocytes, lymphocytes and monocytes. | | | Catalog # | C2446-77B | | Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA and Immunocytochemistry. Other applications not tested. | | Recommended Dilutions | Flow Cytometry: 1:10 | | Immunohistochemistry (Frozen): 1:10. Fix for 10 min in pure acetone and then incubate for 10 min in chloroform. Incubation with a pretested dilution of C2446-77B is advised to be followed an enzyme (alkaline phosphatase) conjugated streptavidin. | | Immunocytochemistry: 1:10. In vitro cultured cells can be fixed with 1% paraformaldehyde. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end either cultured endothelial cells or a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1ng/ml), IL-1 (100U/ml) or LPS (1ug/ml) in tissue culture medium at 37°C. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 6A518 (1G11B1) | | Host | Mouse | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Human CD106. | | Specificity | Recognizes human CD106. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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