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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (Azide Free)

Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified IP IH FC IF
VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein. The monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100kD under reducing conditions and 92kD under nonreducing conditions (1-5).
Catalog #C2446-79AX
Applications Identification and enumeration of CD106 cells by flow cytometry (1-5)
Identification of VCAM-1+ cells by immunofluorescence and immunohistochemical staining (1-5)
Blockage of lymphocyte adhesion in Whitlock-Witte long-term bone marrow cultures (1, 2)
Inhibition of lymphopoiesis in Whitlock-Witte cultures (1, 2)
Immunoprecipitation (1)
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K220
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS. No preservatives added.
PurityPurified immunoglobulin
SpecificityRecognizes CD106/VCAM-1 (~Mr 100kD).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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