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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (PE)

Anti -CD106 (Vascular Cell Adhesion Molecule-1, VCAM-1, INCAM-110) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Purified IP IH FC IF
VCAM-1 is an adhesion molecule and a major mediator of the inflammatory response. It is expressed on activated microvascular endothelial cells in response to signals arising from immune responses in infection, graft rejection, tumor recognition and killing. The complementary binding ligand for VCAM-1 is VLA-4/CD49d. In addition to VCAM-1, VLA-4 also recognizes the extracellular matrix molecule fibronectin. This pairing of VCAM-1 and VLA-4 is able to provide a second signal (i.e., non-antigen specific) for T cell stimulation, such as that seen in transplantation. The monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. In addition to inflammatory responses, VCAM-1 has a significant role in hemopoiesis through its ability to retain lymphocyte and myeloid precursors on stromal cells in the marrow and lymphoid organs. CD106/VCAM-1 exists as an integral membrane protein. The monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100kD under reducing conditions and 92kD under nonreducing conditions (1-5).
Catalog #C2446-79D
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended DilutionFlow Cytometry: <0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityStorage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
Note We do not recommended adding glycerol as a cryopreservative.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No5K220
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% sodium azide and a stabilizing agent. Labeled with PE.
PurityPurified
SpecificityRecognizes mouse CD106/VCAM-1 (~Mr 100kD).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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