Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. May be used for the following: Blockage of lymphocyte adhesion in Whitlock-Witte long-term bone marrow cultures (1, 2), Inhibition of lymphopoiesis in Whitlock-Witte cultures (1, 2) Other applications have not been tested.
Recommended Dilution
Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
BALB/3T3 and +/+2.4 cells
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Purity
Purified
Specificity
Recognizes mouse CD106. Immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100kD under reducing conditions and 92kD under non-reducing conditions.
References
1. Miyake, K., I.L. Weissman, J.S. Greenberger and P. W. Kincade. 1991. J. Exp. Med. 173:599.|2. Miyake, K., et al.. 1991. J. Cell. Biol. 114(3):557.|3. Schlegel, P.G., et al. 1995. J. Immunol. 155:3856.|4. Bergese, S., et al. 1995. Am J. Phathol. 146:989.|5. Orosz, C.G., et al. 1993. Transplantation 56:453.
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