| The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component speci c to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low af nity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity. |
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| Catalog # | C2464-01 |
| Applications | Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA, Immunoprecipitation and Inhibition assays. Other applications not tested. |
| Recommended Dilutions | Flow Cytometry: 1:50 Use 10ul to label 10e6 cells in 100ul. |
| Immunohistochemistry: Frozen |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Spleen cellls from immunized BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line. |
| Recommended Secondary Reagents | I1904-66A IgG, F(ab’)2, X-Adsorbed (FITC) |
| Recommended Negative Controls | I1904-78R: IgG1 Murine Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | N/A |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H1836 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Removal of sodium azide is recommended prior to use in functional assays. |
| Purity | Purified by Ion Exchange chromatography. |
| Immunogen | CHO cells transfected with cDNA of the IL-6 receptor. |
| Specificity | Recognizes soluble and membranous human IL-6R. This antibody has been shown to inhibit IL-6 mediated proliferation of XG-1 cells. It partially blocks binding of IL-6 to its receptor. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
