| Catalog # | C2500-15 |
| Applications | Suitable for use in Western Blot, Immunocytochemistry and FACS Analysis. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.1-0.5ug/ml Detects CEARL in RIPA lysates from HeLa cells. |
| Immunocytochemistry: 1:1000 Reported to show positive immunostaining for CEARL in rat Kupffer cells. |
| FACS Analysis: Reported to detect CEARL in rat Kupffer cells by FACS analysis. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa161-182 [MATTGGMGMGPGGPGMITIPPS] of human carcinoembryonic antigen receptor “long” (CEARL), conjugated to KLH. |
| Specificity | Recognizes human CEARL/hnRNP M4 isoform 1, Mr ~74-80kD. Additional proteins were also detected, Mr ~55 and 70kD. |
| Species Crossreactivity | Mouse and rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
