CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172,
T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor
superfamily member 9 precursor)
Pricing
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| CD137 is an inducible T cell surface protein belonging to the TNF receptor superfamily. The 255aa human CD137 is a type I transmembrane protein having in its extracellular domain four of the cysteine-rich motifs that are characteristic of the TNF receptor superfamily. The 30kD glycoprotein exists both as a monomer and as a dimer on T cells. The human and mouse proteins share 60% aa identity. CD137 is absent from naive T cells, but it is upregulated and continually expressed following T cell activation. The natural ligand, CD137L, is a member of the TNF superfamily and is expressed on activated antigen presenting cells including dendritic cells, macrophages, and B cells. Cross-linking of CD137 by CD137L or by agonistic antibodies transmits a potent co-stimulatory signal that enhances the effect of other activating signals such as PHA or anti-CD3 antibodies. CD137 signals through the TFAF2-NIK pathway resulting in activation of NF-kappaB and ultimately promoting proliferation and survival of T cells. | | | Applications: | | | Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunocytochemistry and Co-stimulation of T cells. Other applications not tested. | | | Recommended Dilutions: | | | Flow Cytometry: 25ug/ml. 10ul of diluted C2548-40E labels 1-2.5x10e5 cells in a total reaction volume 200ul. Validated using PHA-treated T cells. | | | ELISA: 0.2-0.8ug/ml as a capture antibody in a sandwich ELISA in combination with biotinylated human CD137 detection antibody (C2548-40F) and recombinant human CD137 as the standard. | | | Western Blot: 0.1- 0.2ug/ml. The detection limit for recombinant human CD137 is ~0.2ng/lane and 0.5ng/lane under non-reducing and reducing conditions, respectively. | | | Immunocytochemistry: 2-15ug/ml will detect CD137 in cells. | | | Co-stimulation of T cells: 5-20ug/ml stimulates IFN-gamma production of human T cells in the presence of 10ug/ml of monoclonal anti-human CD3. | | | Optimal dilutions to be determined by the researcher. | | | Storage and Stability: | | | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | | Catalog # | C2548-40E | | CAS Number | n/a | | Product Type | Pab | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | ~0.1mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to the extracellular domain of human CD137 expressed in NSO cells. | | Specificity | Recognizes the extracellular domain of human CD137. In ELISA, <0.1% crossreactivity with recombinant mouse CD137, recombinant human NGF R, TNF RI and TNF RII is observed. In Western Blot, shows <1% crossreactivity with recombinant human NGF R, sTNF RI, sTNF RII and recombinant mouse sTNF RI and sTNF RII. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Download Specifications for CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) |
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