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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA, MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor superfamily member 9 precursor) (Biotin)

Anti -CD137 (4-1BB, TNFRSF9, 4-1BB ligand receptor, CD137, CD137 antigen, CDw137, ILA,
MGC2172, T-cell antigen 4-1BB homolog, T-cell antigen ILA, Tumor necrosis factor receptor
superfamily member 9 precursor) (Biotin)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH FC
4-1BB is an inducible T cell surface protein belonging to the TNF receptor superfamily. It is alternatively known as TNFRSF9, CD137, and ILA. The human and mouse proteins share 60% amino acid sequence identity. 4-1BB is absent from naive T cells, but it is upregulated and continually expressed following T cell activation.
Catalog #C2548-40F
ApplicationsSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 2.5ug/10e6 cells using PHA-treated human T cells
ELISA (Capture): 0.2-0.8ug/ml
ELISA (Detection): 0.1-0.4ug/ml
Western Blot: 0.1ug/ml
Immunocytochemistry: 5-15ug/ml using immersion fixed human peripheral blood mononuclear cells
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, BSA. Reconstitute with 250ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant protein corresponding to aa24-186 from human CD137, expressed in NS0 cells (Q07011).
SpecificityRecognizes human CD137. Species Crossreactivity: rmCD137, rhNGF R, rhTNF RI, rhTNF RII; <0.1%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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