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C2548-80P2 Mouse Anti-CD147 (Basigin, 5F7, Collagenase Stimulatory Factor, Extracellular Matrix Metalloproteinase Inducer, EMMPRIN, TCSF, Leukocyte Activation Antigen M6, OK Blood Group Antigen, Tumor Cell-derived Collagenase Stimulatory Factor, UNQ6505/PRO21383)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P35613
Isotype
IgG1,k
Clone Number
HIM6
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu Mk
Shipping Temp
Blue Ice
Storage Temp
-20°C

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Applications
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Flow Cytometry: ≤0.5ug/10e6 cells in 100ul volume or 100ul whole blood Immunohistochemistry (Frozen/FFPE): 0.5-10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human PBMCs
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by affinity chromatography
Specificity
Recognizes human CD147. Species Crossreactivity: chimpanzee, baboon and Capuchin monkey
References
1. Biswas C, et al. 1995. Cancer Res. 55:434. 2. Fadool J, et al. 1993. Dev. Dyn. 196:252. 3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.
USBio References
No references available
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