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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11 Antigen, 5F7, Blood-brain Barrier HT7 Antigen, Collagenase Stimulatory Factor, Emmprin, Leukocyte Activation Antigen M6, M6, OK, OK Blood Group Antigen, TCSF, Tumor Cell Der

Anti -CD147 (Extracellular Matrix Metalloproteinase Inducer, Basigin, Neurothelin, 5A11
Antigen, 5F7, Blood-brain Barrier HT7 Antigen, Collagenase Stimulatory Factor, Emmprin,
Leukocyte Activation Antigen M6, M6, OK, OK Blood Group Antigen, TCSF, Tumor Cell Der

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH FC
Catalog #C2548-81
The antigen is also present on some endothelia [2]. CD147 is a membrane glycoprotein of MW 42-48kD expressed at high levels on activated lymphocytes but lower levels on resting cells. The antigen contains two Ig-like domains and an unusual transmembrane domain with a charged residue [2].It is abundant on activated lymphocytes it is the homologue of the chicken blood brain barrier antigen HT7.
ApplicationsSuitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry (Neat): 1:20. Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Immunohistochemistry (frozen): 1:20
Optimal working dilutions to be determined by researcher.
HybridomaNSO/1 myeloma cells with spleen cells from Balb/c mice.
Recommended Negative ControlI1904-78R: IgG1 Negative Control
Recommended Secondary AntibodyI1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H1875
HostMouse
SourceRat
Concentration~10mg/ml
FormSupplied as a liquid in 0.2M Tris HCl pH 7.4, 5-10% fetal calf serum, 0.09% sodium azide.
PurityTissue Culture Supernatant.
ImmunogenT blasts from a mixed lymphocyte reaction.
SpecificityRecognizes rat CD147.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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