| CD152 is a 45kD glycoprotein, expressed normally as a disulphide-linked homodimer. It is expressed transiently on the surface of T cells following activation (peaking at 3 days), but is often present with the cytoplasm. CD152 is a ligand for CD80 and CD86, and is important in cellular interactions during the immune response. |
|
| Catalog # | C2548-83K |
| Applications | Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: Neat-1:10,.Use 10ul to label 10e6 cells in 100ul. Membrane permeabilisation is required. |
| Immunohistochemistry (Frozen): 5-50ug/ml Not suitable for use on paraffin. |
| Optimal dilution determined by the researcher. |
| Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP |
| F(ab')2 rabbit anti mouse IgG: RPE conjugate |
| Hybridoma | Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line. |
| Recommended Negative Controls | I1904-83P1: IgG2a Mouse Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 3H1883 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.09% sodium azide, 0.5% BSA. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Human CTLA-4/human IgG heavy chain fusion protein. |
| Specificity | Recognizes the human CD152 cell surface antigen, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4). |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
