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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)

Anti -CD152 (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
CD152 is a 45kD glycoprotein, expressed normally as a disulphide-linked homodimer. It is expressed transiently on the surface of T cells following activation (peaking at 3 days), but is often present with the cytoplasm. CD152 is a ligand for CD80 and CD86, and is important in cellular interactions during the immune response.
Catalog #C2548-83K
ApplicationsSuitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat-1:10,.Use 10ul to label 10e6 cells in 100ul. Membrane permeabilisation is required.
Immunohistochemistry (Frozen): 5-50ug/ml Not suitable for use on paraffin.
Optimal dilution determined by the researcher.
Recommended Secondary ReagentsF(ab')2 rabbit anti-mouse IgG HRP
F(ab')2 rabbit anti mouse IgG: RPE conjugate
HybridomaSpleen cells from immunized BALB/c mice were fused with cells of the mouse P3/X63-Ag8.653 myeloma cell line.
Recommended Negative ControlsI1904-83P1: IgG2a Mouse Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No3H1883
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide, 0.5% BSA.
PurityPurified by affinity chromatography.
ImmunogenHuman CTLA-4/human IgG heavy chain fusion protein.
SpecificityRecognizes the human CD152 cell surface antigen, also known as CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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