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You are here:Home » Molecular Biology » MB-CD Markers » CD152, Fc Chimera, Recombinant, Human (CTLA4, Cytotoxic T-lymphocyte-associated Antigen 4)

CD152, Fc Chimera, Recombinant, Human (CTLA4, Cytotoxic T-lymphocyte-associated Antigen 4)

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Specifications

CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization.
Recombinant Human CTLA-4 produced in insect cells is a homodimeric, glycosylated, polypeptide chain containing a total molecular mass of 80kDa. Each subunit (40kDa) is fused to a polypeptide linker to the Fc portion of Human IgG1. CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization.
Recombinant Human CTLA-4 is purified by proprietary chromatographic techniques.
Catalog #C2548-83S
Amino acid sequenceThe sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val.
Dimers and aggregates 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological ActivityRecombinant CTLA-4 is fully biologically active when compared to standards.
Protein contentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280.
2. Analysis by RP-HPLC, using a standard solution of Human CTLA-4 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA. aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight80kD
SourceSf9 Insect cells
Purity95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug) of CTLA-4.
FormSupplied as a lyophilized powder without additives. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. to a concentration of >0.1mg/ml. This solution can then be diluted into other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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