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CD152, Fc Chimera, Recombinant, Human (CTLA4, Cytotoxic T-lymphocyte-associated Antigen 4)
CD152, Fc Chimera, Recombinant, Human (CTLA4, Cytotoxic T-lymphocyte-associated Antigen 4)
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| CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. | | | Recombinant Human CTLA-4 produced in insect cells is a homodimeric, glycosylated, polypeptide chain containing a total molecular mass of 80kDa. Each subunit (40kDa) is fused to a polypeptide linker to the Fc portion of Human IgG1. CTLA-4 is expressed in low copy number by T-cells only after activation, but it binds CD28-ligand with approximately 20-fold higher affinity than CD28. A soluble form of the extracellular domain of CTLA-4 has been shown to bind CD28-ligand with high avidity and to suppress T-cell-dependent antibody responses in vivo. Large doses of this soluble protein also suppress responses to a second immunization. | | | Recombinant Human CTLA-4 is purified by proprietary chromatographic techniques. | | | Catalog # | C2548-83S | | Amino acid sequence | The sequence of the amino acids was determined and was found to be: Lys-Ala-Met-His-Val. | | Dimers and aggregates | 1% as determined by silver-stained SDS-PAGE gel analysis. | | Biological Activity | Recombinant CTLA-4 is fully biologically active when compared to standards. | | Protein content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280. | | 2. Analysis by RP-HPLC, using a standard solution of Human CTLA-4 as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA. aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer | | Molecular Weight | 80kD | | Source | Sf9 Insect cells | | Purity | 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel. Endotoxin: 0.1ng/ug (IEU/ug) of CTLA-4. | | Form | Supplied as a lyophilized powder without additives. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. to a concentration of >0.1mg/ml. This solution can then be diluted into other aqueous solutions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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