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You are here:Home » Molecular Biology » MB-CD Markers » CD152 Human, IgG2a Mouse Fc, Fusion Protein (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (Preservative Free)

CD152 Human, IgG2a Mouse Fc, Fusion Protein (CTLA4, Cytolytic T-lymphocyte-associated
Antigen 4) (Preservative Free)

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Specifications

Immune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human CD152 is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Therefore, CD152 may be an important player in down regulating T cell mediated immune responses
Catalog #C2548-83VX
Molecular Structure A soluble 110kD dimeric fusion protein consisting of the extracellular (125aa) domain of human CTLA-4 fused to murine IgG2a Fc (233aa), with a predicted non glycosylated monomeric molecular weight of 40.1kD.
Transfectant Cell LineBHK
ApplicationsSuitable for use in ELISA and Flow Cytometry. Other applications not tested.
Functional ApplicationBinds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2) (4, 5). Blocks the binding of anti-human CD80 (B7-1) and anti-human CD86 (B7-2) mAbs to recepters on Raji cells. Crossreacts with CD80 and CD86 across a broad range of mammalian species including pig (6).
Recommended DilutionFlow Cytometry:1ug/ml. 80ul of diluted C2548-83VX labels 5x10e5 cultured Raji cells. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight40.1kD
SourceBHK cells
PurityPurified by Protein A affinity chromatography. (> 95% by SDS-PAGE with <1% bovine immunoglobulin)
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, sterile filtered under aseptic conditions. No preservative added.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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