CD153, soluble, Recombinant, Human (CD30 Ligand, CD30L, TNFSF 8)
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| The CD30 ligand (CD30L, CD153) is a member of the tumor necrosis factor (TNF) family. CD30L is a type II, transmembrane protein expressed on T and B cells, monocytes/macrophages, neutrophils, megakaryocytes, erythroid precursors and eosinophils. After interaction with its receptor CD30, CD30L may induce TNF receptor-associated factor 1 (TRAF1), TRAF2, 3 and 5 recruitment. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases. | | | Catalog # | C2548-84K | | MW | ~35kD (SDS-PAGE, under reducing conditions). | | Source | Produced in HEK 293 cells. The extracellular domain of recombinant human CD30L (CD153) (aa63-234) is fused at the N-terminus to a linker peptide (11aa) and a FLAG-tag. | | Reconstitution | Reconstitute with 100ul sterile ddH2O. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein. | | Specificity | rhsCD30L binds to human CD30 (Ki-1). | | Application | Can be used together with an enhancer to detect membrane-bound human CD30. rhsCD30L saturates its receptor at a concentration range of 12-4000ng/ml. | | Swiss-Prot link P32971 | CD30L (human) | | AfCS Signalling Gateway link A000555: CD30L (mouse) | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~35kD | | Purity | ~95% (SDS-PAGE) | | Concentration | ~0.1mg/ml | | Form | Supplied as a lyophilized powder in PBS. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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