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You are here:Home » Molecular Biology » MB-CD Markers » CD153, soluble, Recombinant, Human (CD30 Ligand, CD30L, TNFSF 8)

CD153, soluble, Recombinant, Human (CD30 Ligand, CD30L, TNFSF 8)

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Specifications

The CD30 ligand (CD30L, CD153) is a member of the tumor necrosis factor (TNF) family. CD30L is a type II, transmembrane protein expressed on T and B cells, monocytes/macrophages, neutrophils, megakaryocytes, erythroid precursors and eosinophils. After interaction with its receptor CD30, CD30L may induce TNF receptor-associated factor 1 (TRAF1), TRAF2, 3 and 5 recruitment. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases.
Catalog #C2548-84K
MW ~35kD (SDS-PAGE, under reducing conditions).
SourceProduced in HEK 293 cells. The extracellular domain of recombinant human CD30L (CD153) (aa63-234) is fused at the N-terminus to a linker peptide (11aa) and a FLAG-tag.
ReconstitutionReconstitute with 100ul sterile ddH2O. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SpecificityrhsCD30L binds to human CD30 (Ki-1).
ApplicationCan be used together with an enhancer to detect membrane-bound human CD30. rhsCD30L saturates its receptor at a concentration range of 12-4000ng/ml.
Swiss-Prot link P32971 CD30L (human)
AfCS Signalling Gateway link A000555: CD30L (mouse)
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~35kD
Purity~95% (SDS-PAGE)
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder in PBS.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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