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Specifications

Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function (1). CD154 has been shown to be hyperexpressed by B and T cells in SLE patients (4). CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells and macrophages indicating a possible role for the CD40-CD154 immunoregulatory signaling mechanism during inflammation and immunity in atherogenesis (6).
Catalog #	C2548-84N8
Molecular Structure	A soluble fusion protein consisting of the extracellular domain (213aa) of human CD154 fused to the extracellular domain (167aa) of murine CD8 alpha.
Applications	Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight	42.6kD
Source	CHO cells
Purity	Purified by affinity chromatography.
Concentration	~0.5mg/ml
Form	Supplied as a liquid in 50mM Sodium Phosphate, pH 7.5, 100mM Potassium Chloride, 150mM sodium chloride, 5% Glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.