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You are here:Home » Antibodies » Abs to Receptors, Scavenger » Anti -CD163 (CD163 Antigen, Hemoglobin Scavenger Receptor, M130, M130 Antigen Precursor, Macrophage-associated Antigen, MM130, Scavenger Receptor Cysteine-rich Type 1 Protein M130)

Anti -CD163 (CD163 Antigen, Hemoglobin Scavenger Receptor, M130, M130 Antigen Precursor,
Macrophage-associated Antigen, MM130, Scavenger Receptor Cysteine-rich Type 1 Protein
M130)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP IH FC IF
The CD163 cell surface glycoprotein is a 175kD molecule also known as ED2. CD163 is expressed by ~50% of peritoneal macrophages, a subset of splenic macrophages, and by macrophages in most other tissues. However, it is not expressed by monocytes, alveolar macrophages or microglial cells. May be used in histology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde).
Catalog #C2548-90CD
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence and Immunoprecipitation. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:10-1:100; 10ul labels 10e6 cells in 100ul
Immunohistochemistry (Frozen): 1:50-1:100
Immunohistochemistry (Paraffin): Requires antigen retrieval by protein digestion (trypsin or pronase). Reported reaction following PLP fixation (Periodate-lysine-paraformaldehyde)
Optimal dilutions to be determined by the researcher.
Positive Control for ImmunohistochemistryLiver
HybridomaSP2/0-Ag 14 myeloma cells with spleen cells from BALB/c mice
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypeMonoclonal
IsotypeIgG1
Clone No10B838
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenRat Spleen cell homogenate.
SpecificityRecognizes rat CD163. Does not react with human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCD163 Molecule


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