| CD163 is a membrane glycoprotein of 130kD, which is expressed exclusively on the cell surface of human monocytes and macrophages that predominantly evolve in the late phase of inflammation. The CD163 is a member of the scavenger receptor cysteine-rich superfamily. |
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| Catalog # | C2548-90D2 |
| Applications | Suitable for use in Western Blot, Flow Cytometry, ELISA, Immunoprecipitation, Immunofluorescence, Immunohistochemistry and Functional studies. Other applications not tested. |
| Recommended Dilutions | Western Blot: 1:10. SDS-PAGE under non-denatured conditions. Block blot with 1% skimmed milk for 1h and incubate with C2548-90D2 (0.4ug/ml) in 0.1% BSA/TBS for 1h. Detection of ~130 kD product in spleen extracted in presence of NP-40 or in glucocorticoid-treated human monocytes. (Ref 2,5) |
| Flow Cytometry: 1:10. Permeabilization as well as no permeabilization is used. Block cells with 1% BSA for 30 minutes at 4°C. (Ref 5) |
| Immunohistochemistry (Frozen sections): 1:10 |
| Functional Studies: Reduction of CD69 expression by adding 2.5ug/ml C2548-90D2 to 5x10e5 human T-lymfocytes in presence of exogenous CD163. (Ref 2) |
| Immunofluorescence: Transfected CHO-cells were plated on coverslips and fixed in 4% paraformaldehyde for 20 minutes. Cells were stained with 4ug/ml C2548-90D2 for 60 minutes. |
| Immunoprecipitation: 10ug C2548-90D2 was covalently coupled to beads (with 20 mM dimethyl pimelimidate), blocked with 0.2M glycine buffer for 2 hours and incubated with cell lysate for 2 hours (Ref 5). |
| Optimal dilutions to be determined by the researcher. |
| Recommended Positive Control: |
| CHO-cells transfected with CD163 |
| Recommended Negative Control: |
| Muscle cells |
| Storage and Stability: |
| May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | N/A |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 6A520 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, 0.1% BSA. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Human monocytes |
| Specificity | Recognizes human CD163. Very useful for macrophage phenotyping. Species Crossreactivity: Monkey. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
