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You are here:Home » Antibodies » Abs to CD Markers » Anti -CD235ab (Glycophorin A/B, GpA, GpB)

Anti -CD235ab (Glycophorin A/B, GpA, GpB)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC IF
CD235a (Glycophorin A (GpA)) antigen is exclusively expressed on human erythroid cells and their progenitors. These antibodies may be effectively used for differentiation in acute leukemias e.g. presence of the CD235a antigen on leukemia cells indicates an early erythroid cell lineage of the tumor cells. CD235a antibodies are used to identify erythroid cells during hematopoietic differentiation. CD235a is not expressed on lymphoid or granulocytic progenitor cells and therefore is very useful as a marker for detection of the erythroid cell lineage. CD235a seems to be absent from erythroid colony forming cells in bone marrow, but is present during maturation from pro-erythroid blast cells to mature erythrocytes. CD235a is clinically important in the classification of leukemias. Using Anti-CD235a antibodies, a distinction can be made between erythroleukemia and other (acute) leukemias like e.g. myeloid, lymphoid or undifferentiated leukemias. Anti-CD235a is often used in combination with detection of H-antigen and/or anti-CD36 antibodies for additional characterization of early erythroid cells. Additional immunophenotyping of early erythroleukemias can be performed by detecting myeloid-associated antigens such as CD13, CD14, CD15, CD33 and CD34, to discriminate from lymphoid lineage-associated antigens like CD2, CD7, CD10 and CD19.
Catalog #C2548-98H
ApplicationsSuitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended DilutionsImmunofluorescence: Indirect
Flow Cytometry: 0.015ug/ 100ul peripheral blood
Immunohistochemistry: Staining of acetone-fixed frozen sections and formalin–fixed paraffin sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No5F60
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
SpecificityRecognizes N-terminal, homologous portion of glycophorins A (GPA) and B (GPB) which are single-pass membrane sialoglycoprotein.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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